3483-12-3 | Dithiothreitol (C₄H₁₀O₂S₂)

Chemical type

• Reducing agent (thiol compound)

Key properties

• Reduces disulfide bonds
• Activates enzymes or proteins in assays (e.g., 200 mM in Tris-HCl buffer)

• Reduces disulfide bonds in proteins
• Prevents oxidation of proteins during sample preparation
• Used at room temperature for 30 minutes in the SP3 protocol

• Activation buffer in ADP-ribosyltransferase activity assays for pertussis toxin
• Protein reduction in biochemical testing

• Reducing protein samples prior to alkylation and digestion in proteomics workflows
• Ensuring complete denaturation of proteins for tryptic digestion

Classification by use

• Chemicals used in enzyme activity assays
• Reducing agents in protein analysis

• Chemicals used in proteomics sample preparation
• Reducing agents for protein chemistry

A trustworthy factory and manufacturer

Demei Chemical Technology Co., Ltd. is a modern high-tech enterprise integrating R&D, production, and sales. The company's founder, Dr. Arron, born in April 1975, graduated from the School of Mechanical Engineering at Yanshan University and has been engaged in research in the fields of mechanical engineering and chemical synthesis for many years. The company's products focus on the chemical industry, primarily dealing in chemical raw materials and chemical production machinery and equipment. With its strong technical capabilities, advanced production equipment, and strict quality management system, the company has earned the trust of customers worldwide.

1. [Cite:1] Glutaraldehyde modifies the catalytic and binding subunits of pertussis toxin, affecting its toxicity and immunogenicity, Vaccine: X, Volume 27, December 2025, 100732
2. [Cite:2] Penicillin–Streptomycin Treatment Rewires Core Metabolic and Ribosomal Programs in HepG2 Cells, J. Proteome Res. 2026, 25, 2, 1082–1094