726169-73-9 | Peroxidase (POD)

Chemical type

• Enzyme (Defense-related enzyme)

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• Heme-containing enzyme (EC 1.11.1.x)
• Plant-derived peroxidase isoform from potato leaves (Solanum tuberosum)

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Key properties

• Involved in lignin polymerization and cross-linking of cell wall components.
• Scavenges reactive oxygen species (ROS).

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• Molecular weight: 64 kDa (native, via gel filtration); isoforms ~40-60 kDa (SDS-PAGE)
• Optimal pH: 5.2
• Km values: 0.067 mM for guaiacol, 40 mM for H₂O₂
• Enhanced activity by Zn²⁺ (115.7%) and Ni²⁺ (110.4%); inhibited by Ca²⁺ (34.4%) and Fe²⁺ (61.7%)
• Strongly inhibited by KCN (99.6%), NaN₃ (95.2%), DTT (93.4%), confirming heme-dependent nature
• Specific activity: 705.7 U/mg after purification (11.8-fold purification, 48% recovery)
• Exists as multiple isoforms (3 notable bands on activity gel)

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• Strengthens the cell wall as a physical barrier against pathogen invasion.
• Part of the antioxidant system that mitigates oxidative stress during pathogen attack.

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• Catalyst for oxidation reactions using H₂O₂ and electron donors
• Reporter enzyme in biochemical assays like ELISA, IHC, western blotting, and biosensors
• Component in glucose diagnostic kits for colorimetric detection

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Classification by use

• Defense-related and antioxidant enzyme in plants.

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• Enzymes used in diagnostic kits
• Enzymes used in immunoassays and biosensors
• Plant-derived alternatives to horseradish peroxidase (HRP)

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A trustworthy factory and manufacturer

Demei Chemical Technology Co., Ltd. is a modern high-tech enterprise integrating R&D, production, and sales. The company's founder, Dr. Arron, born in April 1975, graduated from the School of Mechanical Engineering at Yanshan University and has been engaged in research in the fields of mechanical engineering and chemical synthesis for many years. The company's products focus on the chemical industry, primarily dealing in chemical raw materials and chemical production machinery and equipment. With its strong technical capabilities, advanced production equipment, and strict quality management system, the company has earned the trust of customers worldwide.

1. [Cite:1] Integrated transcriptomic and metabolomic analysis of 1-MCP-induced disease resistance in Korla fragrant pear, Current Plant Biology, Volume 45, January 2026, 100576
2. [Cite:2] Isolation and characterization of peroxidase from potato leaves Solanum tuberosum: Application in glucose diagnostic kit, Journal of Genetic Engineering and Biotechnology, Volume 23, Issue 4, December 2025, 100584