Deoxyribonuclease II
CAS: 9025-64-3
Category: Enzyme (Endonuclease, hydrolase)
| Purity | Research Grade (≥90% by SDS-PAGE), Molecular Biology Grade (DNase-free, RNase-free, ≥95%), High-Puri |
| Molecular Formula | Variable |
| Molecular Weight | Variable |
| Appearance | White to off-white lyophilized powder or clear colorless solution (in buffered glycerol or aqueous buffer) |
| Packaging | 1mg/5mg/10mg/25mg/vial (lyophilized); 100 units/500 units/1000 units/vial; 1g/5g/bulk container for industrial use; |
| Testing Indicators | Appearance — Visual inspection; Protein concentration — Bradford assay or UV absorbance (A280); Purity — SDS-PAGE (Coomassie or silver stain); Specific activity — DNA degradation assay (hyperchromicity or gel electrophoresis); Endonuclease activity — DNA substrate gel (zymogram); Protease contamination — Casein or azocollagen assay; RNase contamination — RNA substrate incubation + gel electrophoresis; Endotoxin levels — LAL test; pH optimum profile — Activity assay across pH range; Molecular weight verification — Mass spectrometry (MALDI-TOF or ESI); Isoelectric point — IEF gel; Heavy metals — ICP-MS (for pharmaceutical grade). |
| Storage Notes | Stored as lyophilized powder at -20°C (long-term) or 2–8°C (short-term); reconstituted solutions should be stored at -20°C with added glycerol (50% v/v) to prevent freezing and activity loss; avoid repeated freeze-thaw cycles; protected from light and moisture; away from strong detergents, chelating agents (EDTA inhibits activity), and proteases. |
| Lead Time | Deprecated: htmlspecialchars(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.demeichem.com/config/helpers.php on line 28 days |
Used in molecular biology for the degradation of DNA in the absence of Mg²⁺ (calcium-dependent or acid-optimal activity); for removal of DNA from protein samples and RNA preparations; in apoptosis research (DNase II is involved in DNA fragmentation during programmed cell death in lysosomes); in the preparation of DNA-free recombinant proteins; in forensic DNA analysis; and in pharmaceutical applications for reducing DNA viscosity in biological extracts.