Demei Chemicals Demei Chemicals

YO-PRO-1 (Oxazole yellow iodide)

CAS: 152068-09-2

Category: Organic Intermediates

Purity≥95% (HPLC)
Molecular FormulaC24H29I2N3O
Molecular Weight629.32
AppearanceYellow to orange-brown powder
Packaging1 mg, 5 mg, 10 mg, or 25 mg in amber glass vials; 1 mM solution in DMSO (1 mL)
Testing IndicatorsAppearance — Visual inspection; Purity — HPLC (area normalization); Identification — UV-Vis absorption/fluorescence spectroscopy (λEx 491 nm, λEm 509 nm); Melting point — Capillary method; Moisture — Karl Fischer titration; Solubility — Visual assessment in water/DMSO; Counterion content — Ion chromatography; Mass confirmation — MS or NMR.
Storage NotesStore at 2–8 °C (powder) or –20 °C (DMSO solutions), protected from light, in a dry, airtight container. Desiccate to prevent moisture absorption.
Lead Time10 days
Green-fluorescent carbocyanine monomeric nucleic acid stain used for apoptosis/necrosis detection (cell-impermeant, stains early apoptotic cells), nucleic acid staining in hybridization assays, and cell/virus detection via fluorescence microscopy or flow cytometry. Often combined with propidium iodide (PI) to distinguish live, apoptotic, and necrotic cells.

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