63208-82-2 | Pifithrin-α (PFT-α)

Chemical type

• p53 inhibitor
• Small molecule transcriptional inhibitor of p53

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• Small-molecule inhibitor
• Cyclic hydrobromide form

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Key properties

• Blocks p53 activation and phosphorylation
• Reduces SU11274-induced autophagy and cell death without affecting ERK or Bcl-2 phosphorylation
• Decreases autophagic markers (Beclin-1, LC3-II)
• Used at micromolar concentrations (e.g., 20 µM)

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• Reversible inhibitor of p53-mediated apoptosis and p53-dependent gene transcription (literature description)
• Acts in a p53-independent manner in CRISPR-edited T cells
• Reduces large deletions (>50 bp), chromosomal translocations, and aneuploidy
• Slightly reduces T cell proliferation speed in some loci (e.g., AAVS1)
• Maintains T cell functionality, including cytokine secretion (IFNγ, IL-2, TNF-α), subset composition (naive, effector memory, central memory), and tumor-killing capacity
• Counteracts effects of p53 knockout on deletion sizes
• Potential alternative targets: aryl hydrocarbon receptor, caspase-3, cyclin D1

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• Tool to inhibit p53-mediated pathways in cancer cells
• Agent to study p53's role in autophagy and tumor suppression

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• Addition during CRISPR-Cas9 editing to improve genomic safety of human T cells
• Limits chromosomal aberrations in activated and proliferating T cells
• Enhances safety of CAR T cell products for cancer therapy
• Easily implementable in T cell engineering protocols

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Classification by use

• Chemicals used as transcription factor inhibitors
• Chemicals used in apoptosis and autophagy research

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• Chemicals used in gene editing and CRISPR protocols
• Chemicals for improving safety in adoptive cell therapies
• Chemicals modulating DNA repair outcomes

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A trustworthy factory and manufacturer

Demei Chemical Technology Co., Ltd. is a modern high-tech enterprise integrating R&D, production, and sales. The company's founder, Dr. Arron, born in April 1975, graduated from the School of Mechanical Engineering at Yanshan University and has been engaged in research in the fields of mechanical engineering and chemical synthesis for many years. The company's products focus on the chemical industry, primarily dealing in chemical raw materials and chemical production machinery and equipment. With its strong technical capabilities, advanced production equipment, and strict quality management system, the company has earned the trust of customers worldwide.

1. [Cite:1] Activation of ERK–p53 and ERK-Mediated Phosphorylation of Bcl-2 Are Involved in Autophagic Cell Death Induced by the c-Met Inhibitor SU11274 in Human Lung Cancer A549 Cells, Journal of Pharmacological Sciences, Volume 118, Issue 4, 2012, Pages 423-432
2. [Cite:2] Modulation of TCR stimulation and pifithrin-α improve the genomic safety profile of CRISPR-engineered human T cells, Cell Reports Medicine, Volume 5, Issue 12, 17 December 2024, 101846