Endo-1,3-alpha-glucanase
CAS: 9075-84-7
Category: Carbohydrates
| Purity | Research Grade ≥90% protein content; Enzyme Activity Grade ≥95%; Biotechnology Grade with defined ac |
| Molecular Formula | C30H52N8O15 (representative enzyme composition) |
| Molecular Weight | Approx. 70000-90000 Da (enzyme molecular weight, v |
| Appearance | White to off-white lyophilized powder or pale yellow enzyme preparation; soluble in water; slight characteristic protein odor |
| Packaging | 1 g, 5 g, 10 g lyophilized enzyme vials for laboratory use; 25 kg fiber drums or HDPE containers with moisture-proof inner bags for industrial enzyme preparations |
| Testing Indicators | Appearance — Visual inspection; Protein content — Bradford assay or BCA assay; Enzyme activity — Determination using laminarin or 1,3-alpha-glucan substrate with reducing sugar assay (DNS method); Specific activity — Activity units per mg protein; Identification — SDS-PAGE electrophoresis; Molecular weight — SDS-PAGE and MALDI-TOF MS; Purity — HPLC or electrophoretic analysis; pH optimum and stability — Enzyme activity assay under different pH conditions; Thermal stability — Residual activity measurement after heat treatment. |
| Storage Notes | Store in a cool, dry place away from direct sunlight. Lyophilized enzyme should be stored at 2-8°C for short-term storage and preferably at -20°C for long-term storage. Keep container tightly sealed and protect from moisture. Avoid repeated freeze-thaw cycles. |
| Lead Time | 10 days |
Endo-1,3-alpha-glucanase is a hydrolytic enzyme that selectively cleaves α-1,3-glucosidic linkages in glucans. It is widely used in biochemical research, fungal cell wall degradation studies, enzyme engineering, fermentation processes, food biotechnology, and preparation of oligosaccharides. It is also used as a research reagent for studying polysaccharide metabolism and microbial physiology.