50-52-2 | Thioridazine

Chemical type

• Phenothiazine derivative
• Peroxidase inhibitor (MPO)
• Reversible inhibitor via Compound II accumulation

^[1]

• Phenothiazine antipsychotic drug
• FDA-approved non-antibiotic small molecule

^[2]

Key properties

• Potent inhibitor of PXDN bromination activity (97-99% inhibition at 24 µM)
• IC₅₀ of 1.5 µM in dansylglycine halogenation assay
• Enhances Amplex oxidation without bromide (~50%) but ineffective with bromide
• Good substrate for Compound I but not for Compound II, trapping PXDN in inactive Compound II
• No irreversible inhibition
• Less effective in presence of peroxidase substrates

^[1]

• Moderate binding affinity to CD2068 (Kd ≈ 14.8 µM)
• Inhibits ATPase activity of CD2068 (IC₅₀ ≈ 30 µM)
• Reduces ATP hydrolysis activity significantly
• Antimicrobial activity against multiple Clostridioides difficile ribotypes (MIC 64–128 µg/mL)
• Rapid bactericidal effect (within first hour)
• Additive effect when combined with standard CDI antibiotics
• Capable of modulating gut microbiota composition in vivo

^[2]

• Inhibits PXDN halogenation to modulate oxidative modifications
• Potential for cardiovascular and inflammatory disease models
• Tool for studying peroxidation vs. halogenation cycle diversion

^[1]

• Originally used as an antipsychotic medication
• Investigated as a CD2068 (ABC transporter ATPase) inhibitor
• Adjunct therapy candidate for C. difficile infection (CDI)
• Potential helper compound to enhance antibiotic efficacy
• Potential agent to reverse antibiotic resistance

^[2]

Classification by use

• Reversible enzyme inhibitors
• Inhibitors promoting Compound II accumulation
• MPO-targeted antipsychotics repurposed for peroxidase inhibition

^[1]

• Chemicals used as ABC transporter inhibitors
• Chemicals used to reverse antibiotic resistance

^[2]

A trustworthy factory and manufacturer

Demei Chemical Technology Co., Ltd. is a modern high-tech enterprise integrating R&D, production, and sales. The company's founder, Dr. Arron, born in April 1975, graduated from the School of Mechanical Engineering at Yanshan University and has been engaged in research in the fields of mechanical engineering and chemical synthesis for many years. The company's products focus on the chemical industry, primarily dealing in chemical raw materials and chemical production machinery and equipment. With its strong technical capabilities, advanced production equipment, and strict quality management system, the company has earned the trust of customers worldwide.

1. [Cite:1] Peroxidasin Inhibition by Thioridazine and Other Peroxidase Inhibitors, Antioxidants, 2024, 13(1), 23
2. [Cite:2] Repurposing thioridazine as a potential CD2068 inhibitor to mitigate antibiotic resistance in Clostridioides difficile infection, Computational and Structural Biotechnology Journal, Volume 27, 2025, Pages 887-895